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The periplasmic chaperones associated with the chaperone/usher pathway could possibly be distinguished into two structurally and functionally distinctive subfamilies. Both of these groups vary via the duration of the loop that connects the F1 and G1 β-strands of area one (fourteen).
I, and religated, and also the circular DNA was utilised as matrix for PCR amplification. The DNA fragments So amplified ended up specifically cloned in to the PCR2.1 vector Along with the TA cloning package (Invitrogen) and transformed into E. coli
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